Agilent 1260 Infinity HPLC System with Quat Pump and G4212A DAD

Agilent 1260 Infinity I HPLC System w/ Quat Pump, DAD, & FLD offers the most flexibility for solvent selection and automation in HPLC method development and research, as well as for all HPLC applications requiring continuous access to a wide range of solvents. This system is ideally suited for multi-method, high-throughput workflows and offers convenient access to four solvents for isocratic or gradient analysis, for rapid method development and speeding up preparation of mobile phases and flushing the HPLC system.

The Agilent 1260 Infinity HPLC System’s quaternary pump is based on a two-channel, dual-plunger, in-series design that provides all essential functions of a solvent delivery system. Metering of the solvent and delivery to the high-pressure side are performed by a single pump assembly capable of generating up to 600 bar.

Degassing of the solvents is done in a built-in vacuum degasser integrated into the quaternary pump. The 1260 Infinity Autosampler is designed to offer the well-established flow-through design with variable-volume injection and achieve extremely low carryover, providing increased analysis speed with sensitivity, resolution, and precision. Paired with the autosampler are a thermostatted column compartment and an ALS thermostat, forming a temperature management system that provides heating and cooling to meet the extreme requirements for retention-time reproducibility.

The featured Diode-Array Detector (DAD) is designed for optimal optical performance, GLP compliance, and ease of maintenance. The DAD provides greatly enhanced sensitivity and baseline stability through a cartridge-cell system with optofluidic waveguides. This detector provides a wavelength range of 190-950 nm, a data acquisition rate of up to 80 Hz, and a programmable 1, 2, 4, 8, or 16 nm slit for rapid optimization of sensitivity, linearity, and spectral resolution.

Also featured is the Fluorescence Detector (FLD), which brings high sensitivity and selection through simultaneous multi-wavelength detection. This easy-to-use detector provides quantitative data and fluorescence spectra from a single run. Some of the features of the FLD Detector include: a flash lamp for the highest intensity and lowest detection limit; multi-wavelength mode for on-line spectra; spectra acquisition and simultaneous multi-signal detection; easy front access to the flow cell for fast replacement; and built-in wavelength accuracy verification. Users may also use an optional cuvette for off-line measurements.

Features

• Offers flexibility for solvent selection and automation in HPLC method development and high-throughput workflows
• Features a vacuum degasser integrated into the pump for efficient degassing of solvents
• Quaternary pump accommodates a wide range of solvent compatibilities and mixing capabilities for diverse HPLC applications
• Autosampler designed with a flow-through needle for low carryover and high-speed sample processing
• Programmable slit widths in DAD allow for rapid optimization of detection sensitivity and spectral resolution
• FLD enhances detection with high-intensity flash lamp and multi-wavelength spectral scanning capabilities
• ALS thermostat manages temperature from 4°C to 40°C, ensuring optimal conditions for sensitive analyses
• Column compartment maintains temperature with high stability and accuracy, facilitating reproducible results
• Includes Agilent ChemStation Workstation Software for comprehensive control over data acquisition and analysis
• Enables rapid method development with convenient access to four solvents for isocratic or gradient analysis

Agilent 1260 Infinity I HPLC System w/ Quat Pump, DAD, & FLD Specifications

DAD Detector	Specification
Detection Type	1024-element photodiode array
Light Source	Deuterium and tungsten lamps
Wavelength Range	190 – 640 nm
Short Term Noise (ASTM) Single and Multi-Wavelength	< ± 0.7 × 10^-5 AU at 254 nm and 750 nm
Drift	< 0.9 × 10^-3 AU/hr at 254 nm
Linear Absorbance Range	> 2.0 AU (5%) at 265 nm
Wavelength Accuracy	± 1 nm
Wavelength Bunching	1 – 400 nm
Slit Width	1, 2, 4, 8, 16 nm / programmable slit
Diode Width	< 1 nm
Signal Data Rate	Up to 80 Hz
Spectra Data Rate	Up to 80 Hz
Flow Cell	Standard: 13 µL volume, 10 mm cell path length and 120 bar (1740 psi) pressure maximum
Control and Data Evaluation	Agilent ChemStation for LC
FLD Detector	Specification
Detection Type	Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis
Performance Specifications	Single wavelength operation: • RAMAN (H2O) > 500 (noise reference measured at signal) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell • RAMAN (H2O) > 3000 (noise reference measured at dark value) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell Dual wavelength operation: RAMAN (H2O) > 300 Ex 350 nm, Em 397 nm and Ex 350 nm, Em 450 nm, standard flow cell.
Light source	Xenon Flash Lamp, normal mode 20 W, economy mode 5 W, lifetime 4000 h
Pulse frequency	296 Hz for single signal mode 74 Hz for economy mode
Excitation Monochromator	Range: settable 200 nm – 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 300 nm
Emission Monochromator	Range: settable 200 nm – 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 400 nm
Reference System	In-line excitation measurement
Timetable programing	up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters
Spectrum acquisition	Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200 – 400 nm, 10 nm step) Step size: 1 – 20 nm Spectra storage: All
Wavelength characteristic	Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting
Flow cells	Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz

The Agilent 1260 Infinity I HPLC System w/ Quat Pump, DAD, & FLD can be fully controlled via the included Agilent ChemStation Workstation Software, enabling user-friendly operation for data acquisition, analysis, instrument control, automation, and more. This Agilent system is in excellent condition and will be fully tested to ensure it meets factory specifications before being shipped.

Refurbished Agilent 1260 Infinity HPLC System Sale Includes:

• • Solvent Tray
• • Agilent 1260 Infinity I G1311B- Quat Pump
• • Agilent 1260 Infinity I G1367E- ALS
• • Agilent 1290 Infinity G1330B – Thermostat
• • Agilent 1260  Infinity I G1316A-TCC
• • Agilent 1260 Infinity I G4212A- DAD
• • Windows Pro 11 Workstation loaded with OpenLab
• • LED Monitor
• • Power Cord & communication Cables

Reference

• Brochure