Termociclador de PCR em Tempo Real BioRad CFX96

BioRad CFX96 Real-Time PCR Detection System

BioRad CFX96 Real-Time PCR Thermal Cycler The CFX96 system’s solid-state optical technology (six filtered LEDs, each with a corresponding filtered photodiode) maximizes fluorescence detection for specific dyes in specific channels, providing sensitive detection for precise quantitation and target discrimination.

Scanning just above the sample plate, the optics shuttle individually illuminates and reads fluorescence from each well with high sensitivity and no cross-talk. At every position and with every scan, the optics shuttle is reproducibly centered above each well, so the light path is always optimal and there is no need to sacrifice data collection in one of the channels to normalize to a passive reference.

The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singleplex or multiplex reactions.

Quickly set up runs and monitor amplification traces in real-time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR.

 

Key Features and Benefits

With the CFX96 Touch Real-Time PCR Detection System you can:

• Set up your system quickly — easy installation and factory-calibrated optics
• Minimize sample and reagent use — up to 5-target multiplexing with sample volumes as low as 10 µl
• Optimize reactions in a single run — thermal gradient feature
• Analyze data faster — visualize all run data at once and export only the data you need in the format you want
• Use advanced data analysis tools — normalized gene expression using CFX Maestro Software
• Configure the system to fit your needs — run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX Automation System II for higher throughput
• Combine the CFX96 Touch System with good laboratory practice standards — use CFX Maestro Software, Security Edition for data collection and analysis to simplify compliance with U.S. FDA 21 CFR Part 11 regulations

Flexible CFX System Configurations Meet Your Throughput Needs

O CFX Automation System II works with all CFX Real-Time PCR Detection Systems to enable walk-away, high-throughput qPCR operation. Integrate this robotic plate handler with one or two CFX Systems to maximize throughput while maintaining a compact footprint.

CFX Maestro Software Works Seamlessly with PrimePCR Assays and Panels

The expert design and wet-lab validation of all PrimePCR Assays ensure optimal assay performance. The integration of CFX Maestro Software with PrimePCR Assays aids in the analysis and interpretation of experimental results.

• Begin analyzing data in a single step by importing target, reference, and control information from a PrimePCR run file directly into CFX Maestro Software
• Combine data from multiple plates into a Gene Study to rapidly screen large numbers of targets or samples
• Use powerful data visualization tools, such as hierarchical clustering and color-matched expression levels, to identify individual targets or clusters to consider for further investigation

Service Plans and Validation Tools for CFX Systems, Thermal Cyclers, and Real-Time PCR Instruments

• O Bio-Rad Expert Care Service Program offers various service plans to keep your systems functioning optimally
• CFX System IQ/OQ Services e CFX Automation System II IQ/OQ Services certify system installation and validate system performance
• Thermal Validation Services test temperature accuracy and uniformity
• O CFX Qualification Plate quickly and easily verifies the performance of your CFX Real-Time PCR Detection Systems

SPECIFICATIONS:

Thermal Cycler
Chassis	C1000 Touch™
Maximum ramp rate, °C/sec	5
Average ramp rate, °C/sec	3.3
Heating and cooling method	Peltier
Lid, °C	Heats up to 105
Temperature
Range, °C	0–100
Accuracy, °C	±0.2 of the programmed target at 90°C
Uniformity, °C	±0.4 well-to-well within 10 sec of arrival at 90°C
Gradient
Operational range, °C	30–100
Programmable span, °C	1–24
Optical Detection
Excitação	6 filtered LEDs
Deteção	6 filtered photodiodes
Range of excitation/emission wavelengths, nm	450–730
Sensitivity	Detects 1 copy of the target sequence in human genomic DNA
Gama dinâmica	10 orders of magnitude
Scan Time
All channels, sec	12
FAM/SYBR® Green only, sec	3
Software
Operating systems	Windows 7, Windows 8, Windows 10
Multiplex analysis	Up to 5 targets per well
System
Licensed for real-time PCR	Yes
Sample capacity, wells	96
Sample size, µl	1–50 (10–25 recommended)
Communication interface	USB 2.0
Electrical approvals	IEC, CE
Dimensions (W x D x H), cm/in	33 x 46 x 36 /13 x 18 x 14
Weight, kg/lb	21/47

Components:

• CFX96 Optical Reaction Module (96-Well)
• USB Cable
• PC installed with Bio-Rad CFX Manager software
• C1000 Touch Thermal Cycler Chassis
• USB cable
• PC installed with Bio-Rad CFX Maestro Software
• Instruction manual
• qbase+ Software license
• SsoAdvanced™ Universal SYBR^® Green Supermix (1725270)
• Hard-Shell^® Low-Profile Thin-Wall 96-Well Skirted PCR Plates (HSP9601)
• Microseal^® ‘B’ Adhesive Seals (MSB1001)

Biblioteca de Referência

BioRad CFX96 Real-Time PCR Thermal Cycler Manufacturer Information
BioRad CFX96 Real-Time PCR Thermal Cycler Brochure