{"id":2387,"date":"2020-07-28T11:09:15","date_gmt":"2020-07-28T11:09:15","guid":{"rendered":"https:\/\/sonoransurplus.com\/?post_type=product&#038;p=2387"},"modified":"2024-09-03T17:36:18","modified_gmt":"2024-09-03T17:36:18","slug":"lightcycler-480","status":"publish","type":"product","link":"https:\/\/sonoransurplus.com\/pt\/product\/lightcycler-480\/","title":{"rendered":"LightCycler 480 Instrument II da Roche"},"content":{"rendered":"<h2 class=\"x-h3 x-spacing-medium\" style=\"text-align: justify;\">Unleash the potential of real-time PCR<\/h2>\n<div class=\"x-text\" style=\"text-align: justify;\">\n<h3><b>Proven, high performance<\/b><\/h3>\n<p>The LightCycler<sup>\u00a0<\/sup>480 System is a proven high-performance, medium-to high-throughput PCR platform that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. Benefit from the cutting-edge thermal block and optical technology that leads to excellent well-to-well homogeneity and maximized detection sensitivity. Generate meaningful results via the state-of-the-art data analysis methods, in line with MIQE guidelines, and analyze true raw data without the need for passive reference dyes or normalization plates.<\/p>\n<style>.embed-container { position: relative; padding-bottom: 56.25%; height: 0; overflow: hidden; max-width: 100%; } .embed-container iframe, .embed-container object, .embed-container embed { position: absolute; top: 0; left: 0; width: 100%; height: 100%; }<\/style>\n<div class=\"embed-container\"><iframe src=\"https:\/\/www.youtube.com\/embed\/0rCxdtlXNj8\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/div>\n<p>&nbsp;<\/p>\n<h3><b>The ideal combination of speed, accuracy, and versatility<\/b><\/h3>\n<p>The LightCycler<sup>\u00ae<\/sup> 480 System features a versatile, plate-based, real-time PCR device that supports mono- or multicolor applications. The benchtop instrument is easily customizable to meet changing user requirements and can be integrated into everyday use as an automated high-throughput solution.<\/p>\n<\/div>\n<p style=\"text-align: justify;\">LightCycler 480 II is a real-time PCR cycler for mid to high sample throughput. The LightCycler 480 II with LED is suitable for detection systems with different probe types such as hybridization probes or hydrolysis (5\u2019Nuclease \/ TaqMan) probes. With a total of 6 detection channels, this cycler offers the highest flexibility and allows single color and multiplex testing.<\/p>\n<p style=\"text-align: justify;\">The LightCycler 480 Instrument II is a rapid, plate-based, thermal block cycler with integrated real-time, online detection capabilities. Based on the comprehensive improvement of the Peltier-based technology incorporated in a silver block, the instrument provides extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility. The special arrangement of optical components in the<\/p>\n<p style=\"text-align: justify;\">LightCycler 480 Instrument II ensures the uniform collection of signals across the plate and makes analysis independent of the sample position on the plate. The LightCycler 480 System setup enables the use of all current probe formats (e.g., SYBR Green I, ResoLight dye for high-resolution melting, hydrolysis probes, HybProbe probes, SimpleProbe probes) and provides the ideal solution for fast and precise qualitative or quantitative detection of nucleic acids, genotyping, and mutation scanning. Channels and filters can be combined in different ways to allow flexibility in mono-color as well as multiplex assays.<\/p>\n<p style=\"text-align: justify;\">The LightCycler 480 Software provides an intuitive, easy-to-navigate user interface for real-time PCR programming, data capture, and data analysis. It facilitates highly versatile and extensive data analysis to study gene expression and genetic variation. The LightCycler\u00ae 480 Software enables users to quickly customize the system to meet different application requirements, from basic manual analysis to automated evaluation of data using the software macro feature.<\/p>\n<p style=\"text-align: justify;\">The LightCycler<sup>\u00ae<\/sup>\u00a0480 System is a high-performance, medium- to high-throughput PCR platform (96- or 384-well plates) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. The system features the LightCycler<sup>\u00ae<\/sup> 480 Instrument, a versatile, plate-based real-time PCR device that supports mono- or multicolor applications, as well as multiple protocols. The benchtop instrument is easily customizable to meet changing user requirements and can be integrated into everyday use as a robotically controlled, automated high-throughput solution. The LightCycler<sup>\u00ae<\/sup>\u00a0480 Instrument is designed for general laboratory use and is not intended for use in diagnostic procedures.<\/p>\n<ul style=\"text-align: justify;\">\n<li><b>Obtain fast, highly sensitive, and reproducible\u00a0results\u00a0<\/b>for qualitative and quantitative detection of nucleic acids, mutation scanning, and SNP analysis. Use basic and advanced methods for gene expression and genotyping based on endpoint analysis or melting curves.<\/li>\n<li><b>Benefit from the cutting-edge thermal block and optical technology<\/b>\u00a0that leads to excellent well-to-well homogeneity and maximized detection sensitivity.<\/li>\n<li><b>Use a wide range of probe formats<\/b>\u00a0(<i>e.g.,<\/i>\u00a0FRET or HybProbe probes, SimpleProbe probes, hydrolysis probes) and fluorescent dyes (<i>e.g.,<\/i>\u00a0SYBR Green I, ResoLight Dye, FAM, HEX, Cy5, LightCycler<sup>\u00ae<\/sup>\u00a0Red 610, LightCycler<sup>\u00ae<\/sup>\u00a0Red 640, and LightCycler<sup>\u00ae<\/sup>\u00a0CYAN 500).<\/li>\n<li><b>Choose the optimal type of multiwell\u00a0plate\u00a0<\/b>(white or clear) to best fit your assay format, application, and workflow. Use the available adapter plate to also work with LightCycler<sup>\u00ae<\/sup>\u00a0tube strips.<\/li>\n<li><b>Build a modular real-time PCR platform\u00a0to meet your particular needs.<\/b>\u00a0Flexibly switch between plate formats and thermal block cyclers in just a few minutes with no recalibration time, and benefit from additional user-friendly software modules.<\/li>\n<li><b>Extract meaningful results via the state-of-the-art data analysis methods,<\/b>\u00a0in line with MIQE guidelines. Analyze true raw data without the need for passive reference dyes or normalization plates.<\/li>\n<li><b>Protect your data<\/b>\u00a0using a system compatible with 21 CFR part 11 requirements.<\/li>\n<li><b>Integrate the LightCycler<sup>\u00ae<\/sup>\u00a0480 System as part of a LIMS<\/b>\u00a0in combination with robotic pipetting and plate loading.<\/li>\n<\/ul>\n<p style=\"text-align: justify;\"><span class=\"headline3\"><b>Contents<\/b><\/span><\/p>\n<ul style=\"text-align: justify;\">\n<li>LightCycler<sup>\u00ae<\/sup>\u00a0480 Instrument, 96- or 384-well<\/li>\n<li>LightCycler<sup>\u00ae<\/sup>\u00a0480 Block Cycler Unit, 96- or 384-well<\/li>\n<li>LightCycler<sup>\u00ae<\/sup>\u00a0480 Software*<\/li>\n<li>LightCycler<sup>\u00ae<\/sup>\u00a0480 Instrument Operator\u2019s Manual<\/li>\n<li>LightCycler<sup>\u00ae<\/sup>\u00a0480 LED Lamp (Spare Lamp)<\/li>\n<li>LightCycler<sup>\u00ae<\/sup>\u00a0480 Multiwell Plates, 96- or 384-well, white<\/li>\n<li>Pentium Desktop PC<\/li>\n<\/ul>\n<p style=\"text-align: justify;\">** including absolute quantification, T<sub>m<\/sub>\u00a0calling, relative quantification, melting-curve-based genotyping, and endpoint genotyping<\/p>\n<h2 style=\"text-align: justify;\">INCLUDE:<\/h2>\n<ul style=\"text-align: justify;\">\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Roche<\/span>\n<ul>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Light Cycler 480 II \/ 384<\/span><\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">96-well kit currently installed<\/span><\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Included 384-well kit<\/span><\/li>\n<\/ul>\n<\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Dimensions:<\/span>\n<ul>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">60 cm x 60 cm x 54.5 cm (24 in x 24 in x 21.5 in) (W x D x H)<\/span><\/li>\n<\/ul>\n<\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Weight:<\/span>\n<ul>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Approximately 121lbs<\/span><\/li>\n<\/ul>\n<\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Sample Number:<\/span>\n<ul>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">96 or 384<\/span><\/li>\n<\/ul>\n<\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Reaction Vessel:<\/span>\n<ul>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">96-well PCR plate, 384-well PCR plate<\/span><\/li>\n<\/ul>\n<\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Reaction Volume:<\/span>\n<ul>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">96-well PCR plate: 10 &#8211; 100 \u03bcL; 384-well PCR plate: 5 &#8211; 20 \u03bcL<\/span><\/li>\n<\/ul>\n<\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Excitation Source:<\/span>\n<ul>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Broad-spectrum, high-intensity LED (390 \u2013 710 nm)<\/span><\/li>\n<\/ul>\n<\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Excitation Filters:<\/span>\n<ul>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">440, 465, 498, 533, 618 nm (System II)<\/span><\/li>\n<\/ul>\n<\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Detection:<\/span>\n<ul>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">CCD camera<\/span><\/li>\n<\/ul>\n<\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Detection Filters:<\/span>\n<ul>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">488, 510, 580, 610,640, 660 nm (System II)<\/span><\/li>\n<\/ul>\n<\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Measuring Mode:<\/span>\n<ul>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Real-time, online (continuous display of readings)<\/span><\/li>\n<\/ul>\n<\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Temperature Control:<\/span>\n<ul>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Peltier-based heating\/cooling<br \/>\nRun Time : &lt;40 minutes (384-well PCR plate)<\/span>\u200b<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul style=\"text-align: justify;\">\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Gene Quantification<\/span><\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Gene detection<\/span><\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Absolute quantification<\/span><\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Relative quantification\/gene expression analysis<\/span><\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Genetic Variation Analysis<\/span><\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Melt-curve genotyping with labeled probes<\/span><\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Endpoint genotyping<\/span><\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">High-Resolution Melting (HRM)<\/span><\/li>\n<li><span style=\"color: #000000; font-family: times new roman, times, serif;\">Multiple Plate Analysis<\/span><\/li>\n<\/ul>\n<h2>Reference Library<\/h2>\n<p><strong><a href=\"https:\/\/lifescience.roche.com\/global_en\/products\/lightcycler14301-480-instrument-ii.html\" target=\"_blank\" rel=\"noopener noreferrer\">Roche LightCycler 480 Instrument II Manufacturer Information<\/a><\/strong><br \/>\n<strong><a href=\"https:\/\/sonoransurplus.com\/wp-content\/uploads\/2020\/07\/LightCycler-480-brochure.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Roche LightCycler 480 Instrument II Brochure<\/a><\/strong><\/p>","protected":false},"excerpt":{"rendered":"<p style=\"text-align: justify;\">LightCycler 480 II is a high-performance, medium- to high-throughput PCR platform (96-well plates) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation.<\/p>","protected":false},"featured_media":2389,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_cat":[235],"product_tag":[243],"class_list":["post-2387","product","type-product","status-publish","has-post-thumbnail","product_cat-pcr-machine","product_tag-lightcycler-480","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/sonoransurplus.com\/pt\/wp-json\/wp\/v2\/product\/2387","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sonoransurplus.com\/pt\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sonoransurplus.com\/pt\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/sonoransurplus.com\/pt\/wp-json\/wp\/v2\/comments?post=2387"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sonoransurplus.com\/pt\/wp-json\/wp\/v2\/media\/2389"}],"wp:attachment":[{"href":"https:\/\/sonoransurplus.com\/pt\/wp-json\/wp\/v2\/media?parent=2387"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sonoransurplus.com\/pt\/wp-json\/wp\/v2\/product_cat?post=2387"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sonoransurplus.com\/pt\/wp-json\/wp\/v2\/product_tag?post=2387"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}